High and novel genetic diversity of Francisella tularensis in Germany and indication of environmental persistence.
Identifieur interne : 000030 ( Main/Exploration ); précédent : 000029; suivant : 000031High and novel genetic diversity of Francisella tularensis in Germany and indication of environmental persistence.
Auteurs : C. Schulze [Allemagne] ; K. Heuner [Allemagne] ; K. Myrtenn S [Suède] ; E. Karlsson [Suède] ; D. Jacob [Allemagne] ; P. Kutzer [Allemagne] ; K. Gro E [Allemagne] ; M. Forsman [Suède] ; R. Grunow [Allemagne]Source :
- Epidemiology and infection [ 1469-4409 ] ; 2016.
Abstract
In Germany tularemia is a re-emerging zoonotic disease. Therefore, we investigated wild animals and environmental water samples for the presence and phylogenetic diversity of Francisella tularensis in the poorly studied Berlin/Brandenburg region. The phylogenomic analysis of three isolates from wild animals revealed three new subclades within the phylogenetic tree of F. tularensis [B.71 from a raccoon dog (Nyctereutes procyonoides); B.74 from a red fox (Vulpes vulpes), and B.75 from a Eurasian beaver (Castor fiber albicus)]. The results from histological, PCR, and genomic investigations on the dead beaver showed that the animal suffered from a systemic infection. Indications were found that the bacteria were released from the beaver carcass into the surrounding environment. We demonstrated unexpectedly high and novel phylogenetic diversity of F. tularensis in Germany and the fact that the bacteria persist in the environment for at least one climatic season. These findings support a broader host species diversity than previously known regarding Germany. Our data further support the assumption derived from previous serological studies of an underestimated frequency of occurrence of the pathogen in the environment and in wild animals. F. tularensis was isolated from animal species not previously reported as natural hosts in Germany.
DOI: 10.1017/S0950268816001175
PubMed: 27356883
Affiliations:
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Schulze</name><affiliation wicri:level="1"><nlm:affiliation>Landeslabor Berlin-Brandenburg,Frankfurt (Oder),Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Landeslabor Berlin-Brandenburg,Frankfurt (Oder)</wicri:regionArea><wicri:noRegion>Frankfurt (Oder)</wicri:noRegion><wicri:noRegion>Frankfurt (Oder)</wicri:noRegion><wicri:noRegion>Frankfurt (Oder)</wicri:noRegion></affiliation></author><author><name sortKey="Heuner, K" sort="Heuner, K" uniqKey="Heuner K" first="K" last="Heuner">K. 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Jacob</name><affiliation wicri:level="3"><nlm:affiliation>Robert Koch Institute, Centre for Biological Threats and Special Pathogens,Division 2 (ZBS 2),Highly Pathogenic Microorganisms,Berlin,Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Robert Koch Institute, Centre for Biological Threats and Special Pathogens,Division 2 (ZBS 2),Highly Pathogenic Microorganisms,Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kutzer, P" sort="Kutzer, P" uniqKey="Kutzer P" first="P" last="Kutzer">P. Kutzer</name><affiliation wicri:level="1"><nlm:affiliation>Landeslabor Berlin-Brandenburg,Frankfurt (Oder),Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Landeslabor Berlin-Brandenburg,Frankfurt (Oder)</wicri:regionArea><wicri:noRegion>Frankfurt (Oder)</wicri:noRegion><wicri:noRegion>Frankfurt (Oder)</wicri:noRegion><wicri:noRegion>Frankfurt (Oder)</wicri:noRegion></affiliation></author><author><name sortKey="Gro E, K" sort="Gro E, K" uniqKey="Gro E K" first="K" last="Gro E">K. Gro E</name><affiliation wicri:level="1"><nlm:affiliation>Stadt Brandenburg an der Havel,Veterinär- und Lebensmittelüberwachungsamt,Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Stadt Brandenburg an der Havel,Veterinär- und Lebensmittelüberwachungsamt</wicri:regionArea><wicri:noRegion>Veterinär- und Lebensmittelüberwachungsamt</wicri:noRegion><wicri:noRegion>Veterinär- und Lebensmittelüberwachungsamt</wicri:noRegion><wicri:noRegion>Veterinär- und Lebensmittelüberwachungsamt</wicri:noRegion></affiliation></author><author><name sortKey="Forsman, M" sort="Forsman, M" uniqKey="Forsman M" first="M" last="Forsman">M. Forsman</name><affiliation wicri:level="1"><nlm:affiliation>CBRN Defence and Security,Swedish Defence Research Agency (FOI),Umeå,Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>CBRN Defence and Security,Swedish Defence Research Agency (FOI),Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Grunow, R" sort="Grunow, R" uniqKey="Grunow R" first="R" last="Grunow">R. Grunow</name><affiliation wicri:level="3"><nlm:affiliation>Robert Koch Institute, Centre for Biological Threats and Special Pathogens,Division 2 (ZBS 2),Highly Pathogenic Microorganisms,Berlin,Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Robert Koch Institute, Centre for Biological Threats and Special Pathogens,Division 2 (ZBS 2),Highly Pathogenic Microorganisms,Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j">Epidemiology and infection</title><idno type="eISSN">1469-4409</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In Germany tularemia is a re-emerging zoonotic disease. Therefore, we investigated wild animals and environmental water samples for the presence and phylogenetic diversity of Francisella tularensis in the poorly studied Berlin/Brandenburg region. The phylogenomic analysis of three isolates from wild animals revealed three new subclades within the phylogenetic tree of F. tularensis [B.71 from a raccoon dog (Nyctereutes procyonoides); B.74 from a red fox (Vulpes vulpes), and B.75 from a Eurasian beaver (Castor fiber albicus)]. The results from histological, PCR, and genomic investigations on the dead beaver showed that the animal suffered from a systemic infection. Indications were found that the bacteria were released from the beaver carcass into the surrounding environment. We demonstrated unexpectedly high and novel phylogenetic diversity of F. tularensis in Germany and the fact that the bacteria persist in the environment for at least one climatic season. These findings support a broader host species diversity than previously known regarding Germany. Our data further support the assumption derived from previous serological studies of an underestimated frequency of occurrence of the pathogen in the environment and in wild animals. F. tularensis was isolated from animal species not previously reported as natural hosts in Germany.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Suède</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Schulze, C" sort="Schulze, C" uniqKey="Schulze C" first="C" last="Schulze">C. Schulze</name></noRegion><name sortKey="Gro E, K" sort="Gro E, K" uniqKey="Gro E K" first="K" last="Gro E">K. Gro E</name><name sortKey="Grunow, R" sort="Grunow, R" uniqKey="Grunow R" first="R" last="Grunow">R. Grunow</name><name sortKey="Heuner, K" sort="Heuner, K" uniqKey="Heuner K" first="K" last="Heuner">K. Heuner</name><name sortKey="Jacob, D" sort="Jacob, D" uniqKey="Jacob D" first="D" last="Jacob">D. Jacob</name><name sortKey="Kutzer, P" sort="Kutzer, P" uniqKey="Kutzer P" first="P" last="Kutzer">P. Kutzer</name></country><country name="Suède"><noRegion><name sortKey="Myrtenn S, K" sort="Myrtenn S, K" uniqKey="Myrtenn S K" first="K" last="Myrtenn S">K. Myrtenn S</name></noRegion><name sortKey="Forsman, M" sort="Forsman, M" uniqKey="Forsman M" first="M" last="Forsman">M. Forsman</name><name sortKey="Karlsson, E" sort="Karlsson, E" uniqKey="Karlsson E" first="E" last="Karlsson">E. Karlsson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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